文章基本信息

标题：DNA gyrase: an enzyme that introduces superhelical turns into DNA
本地全文：下载
作者：M Gellert ; K Mizuuchi ; M H O'Dea 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1976
卷号：73
期号：11
页码：3872-3876
DOI：10.1073/pnas.73.11.3872
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Relaxed closed-circular DNA is converted to negatively supercoiled DNA by DNA gyrase. This enzyme has been purified from Escherichia coli cells. The reaction requires ATP and Mg++ and is stimulated by spermidine. The enzyme acts equally well on relaxed closed-circular colicin E1, phage lambda, and simian virus 40 DNA. The final superhelix density of the DNA can be considerably greater than that found in intracellularly supercoiled DNA.