文章基本信息

标题：lac repressor: 3-fluorotyrosine substitution for nuclear magnetic resonance studies
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作者：P Lu ; M Jarema ; K Mosser 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1976
卷号：73
期号：10
页码：3471-3475
DOI：10.1073/pnas.73.10.3471
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：This paper describes the isolation of 3-fluorotyrosine-substituted lac repressor, and its 19F nuclear magnetic resonance spectrum. From the spectrum, one can conclude that for each of the four identical subunits of the repressor there are four or five surface tyrosines, two buried or internal tyrosines, and one tyrosine with an phenolic group ionized or involved in a hydrogen bond. Conditions are described that can be used for the 3-fluorotyrosine substitution of a variety of Escherichia coli proteins for 19F nuclear magnetic resonance studies.