文章基本信息

标题：DNA dependent synthesis of protein L12 from escherichia coli ribosomes, in vitro
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作者：F Chu ; H F Kung ; P Caldwell 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1976
卷号：73
期号：9
页码：3156-3159
DOI：10.1073/pnas.73.9.3156
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The in vitro synthesis of ribosomal protein L12 has been obtained in a coupled system with DNA extracted from the transducing phage lambdarifd18 as template. In addition, a second protein (molecular weight of 16,000) with immunological, chemical, and ribosome-binding characteristics similar to L12 is formed in this in vitro system. The synthesis of both proteins is depressed by one-half when guanosine-5'-diphosphate-3'-diphosphate is added to the reaction mixture.