文章基本信息

标题：Lymphocyte stimulation: a rapid multiparameter analysis
本地全文：下载
作者：Z Darzynkiewicz ; F Traganos ; T Sharpless 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1976
卷号：73
期号：8
页码：2881-2884
DOI：10.1073/pnas.73.8.2881
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Several parameters of stimulation of individual lymphocytes are measured simultaneously by flow-cytofluorometry after differential staining of cellular DNA and RNA with the metachromatic fluorescent dye acridine orange. The method provides a means of analyzing the progression of stimulated cells through the cell cycle (G1, S and G2 + M), in addition to measuring the accumulation of RNA per cell; the RNA parameter is useful in distinguishing G1 from G0 cells. The multiparameter analysis may be of unique value in discriminating cases where the transcriptional and proliferative responses of lymphocytes to stimulants are not correlated.