文章基本信息

标题：Decreased fidelity of DNA polymerase activity isolated from aging human fibroblasts
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作者：S Linn ; M Kairis ; R Holliday 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1976
卷号：73
期号：8
页码：2818-2822
DOI：10.1073/pnas.73.8.2818
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：DNA polymerase (deoxynucleosidetriphosphate: DNA nucleotidyltransferase, EC 2.7.7.7 or DNA nucleotidyltransferase) activity, isolated from late and early passage cells of the diploid human fibroblast line, MRC-5, was compared. The level of activity dropped with increasing passage. In addition, when the fidelity of polymerization was monitored with four synthetic templates under a variety of conditions, it was observed that the enzyme from late passage cells was more error-prone. The possible relation of these observations to "senescence" of the fibroblasts is discussed.