文章基本信息

标题：Isolation and characterization of the protein coded by gene A of bacteriophage phiX174 DNA
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作者：J E Ikeda ; A Yudelevich ; J Hurwitz 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1976
卷号：73
期号：8
页码：2669-2673
DOI：10.1073/pnas.73.8.2669
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Replication of phiX174 circular replicative form (RFI) DNA by extracts of Escherichia coli infected with bacteriophage phiX174 (amber in gene A) requires the phiX174 gene A product. This requirement has been used as an assay for the isolation of this protein. The gene A product (purified 4000-fold) caused relaxation of superhelical phiX174 RFI and formation of discontinuities in the viral strand of phiX174 RFI uniquely situated in the A region of the genome, and yielded a complex after interacting with phiX174 RFI that is active in replication of phiX RFI.