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标题：Paradoxical effects of cycloheximide and cytochalasin B on hamster cell hexose uptake
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作者：C W Christopher ; D Ullrey ; W Colby 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1976
卷号：73
期号：7
页码：2429-2433
DOI：10.1073/pnas.73.7.2429
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Cellular regulation of hexose uptake was studied in cultures of NIL hamster cells. Enhancements of galactose uptake were elicited most strikingly by maintaining confluent NIL cultures in culture media devoid of glucose. These glucose-starved cultures showed up to 8- or 9-fold enhancements in the galactose uptake test. When these cultures were treated for extended periods with cycloheximide, the enhanced uptake was left unimpaired, whereas the uptake by glucose-fed cells, similarly treated with cycloheximide, was inhibited greater than 90%. Addition of glucose to these starved cultures resulted in a gradual decline of uptake rates to the unenhanced level (t1/2 approximately 3 hr). In surprising contrast, when both glucose and cycloheximide were added simultaneously, the decline was arrested for at least 12 hr. If cytochalasin B (the specific inhibitor of hexose transport) was present, the uptake of galactose by both starved and fed cells was close to completely inhibited. By several criteria, cells maintained for 24 hr in medium containing both glucose and cytochalasin B were glucose-fed. Yet, when the cytochalasin B was removed, the cells were found to have enhanced rates of galactose uptake. The regulation of the hexose uptake system may therefore not be guided by the levels of glucose catabolites. Alternative mechanisms that may control hexose uptake are considered.