文章基本信息

标题：Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities
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作者：D L Kacian ; J C Myers
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1976
卷号：73
期号：7
页码：2191-2195
DOI：10.1073/pnas.73.7.2191
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The synthesis of large, possibly complete, complementary DNA (cDNA) copies of poliovirus RNA by avian myeloblastosis virus DNA polymerase is described. The cDNA consists of two size classes, the larger of which is approximately 7500 nucleotides. In the presence of excess deoxynucleoside triphosphates, ribonucleoside triphosphates, or sodium pyrophosphate, only the larger material is obtained. Yields of the large cDNA are 50-75% of the input RNA.