文章基本信息

标题：In vitro transcription of chromatin in the presence of a mercurated nucleotide
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作者：G F Crouse ; J B Fodor ; P Doty 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1976
卷号：73
期号：5
页码：1564-1567
DOI：10.1073/pnas.73.5.1564
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Mercurated uridine triphosphate has been used to label transcripts of chicken reticulocyte chromatin made with Escherichia coli RNA polymerase. The mercury-labeled RNA product can be completely separated from endogenous RNA sequences in the chromatin by passage through a sulfhydryl Sepharose column. Globin cDNA hybridization to the transcript shows that only 2.6 x 10-5 of the transcript is globin RNA. In contrast to this result, erythrocte chromatin transcript contains less than one tenth as many globin RNA sequences.