文章基本信息

标题：Synthesis of acetylcholine receptor during differentiation of cultured embryonic muscle cells
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作者：J P Merlie ; A Sobel ; J P Changeux 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1975
卷号：72
期号：10
页码：4028-4032
DOI：10.1073/pnas.72.10.4028
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Acetylcholine receptor, a component of the specialized muscle membrane, appears during the differentiation of embryonic myogenic cells in tissue culture. Demonstration of incorporation of the radioactive precursor L-[35S]methionine into purified receptor polypeptides is presented as evidence for its synthesis de novo. The identity of the purified radioactive species is established by cosedimentation of the [35S]receptor with [3H]alpha-toxin binding activity on sucrose gradients and by crossreaction with antiserum to purified acetylcholine receptor of Electrophorus electricus.