文章基本信息

标题：Synthesis of RNA containing a methylated blocked 5′ terminus by HeLa nuclear homogenates
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作者：Yoram Groner ; Jerard Hurwitz
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1975
卷号：72
期号：8
页码：2930-2934
DOI：10.1073/pnas.72.8.2930
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：RNA synthesized in vitro by HeLa nuclei contains a variety of blocked methylated 5'-terminal structures that can be grouped into four general types: (a) m7GppNmp...; (b) m7GppNmpMmp...; (c) m7GpppNmp...; and (d) m7GpppNmpMm.... Both Nm and Mm can be either one of the four 2'-O-methylated nucleosides. The relative amounts of the Nm species are G = 45%, A = 25%, U = 20%, and C = 10%.
关键词：5′-blocked methylated RNA ; nuclear lysate ; in vitro synthesis