首页    期刊浏览 2024年12月05日 星期四
登录注册

文章基本信息

  • 标题:RNAs of simian virus 40 in productively infected monkey cells: kinetics of formation and decay in enucleate cells
  • 本地全文:下载
  • 作者:Y Aloni ; M Shani ; Y Reuveni
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1975
  • 卷号:72
  • 期号:7
  • 页码:2587-2591
  • DOI:10.1073/pnas.72.7.2587
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:We demonstrate here the usefulness of cytochalasin B enucleate cells for the study of the metabolism of cytoplasmic mRNA and for determining its half-life in animal cells. Simian virus 40 infected monkey cells in which the RNA had been labeled with [3H]uridine were enucleated, and the decay of the two prominent RNAs of simian virus 40, the 19S and 16S species, was measured by analysis on sucrose gradients. The results of these experiments, together with kinetic analysis of nuclear and cytoplasmic viral RNA, indicate a precursor-product relationship between the 19S and 16S cytoplasmic viral RNA species, which decay by first-order kinetics with a mean half-life of about 3 hr and 6 hr, respectively.
国家哲学社会科学文献中心版权所有