文章基本信息

标题：Deoxysubstitution in RNA by RNA Polymerase In Vitro: A New Approach to Nucleotide Sequence Determinations
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作者：Gary V. Paddock ; Howard C. Heindell ; Winston Salser 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：12
页码：5017-5021
DOI：10.1073/pnas.71.12.5017
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Deoxynucleotides have been incorporated into RNA synthesized in vitro by RNA polymerase with either double-stranded or single-stranded DNA as a template. By use of this technique to block or promote cleavage at a particular phosphodiester bond, a variety of specific cleavages may be obtained with the available ribonucleases and deoxyribonuclease I. These methods should greatly increase the ease and rapidity of nucleotide sequence determinations.
关键词：pancreatic DNase I ; U1 ribonuclease ; U2 ribonuclease ; pancreatic ribonuclease A ; ribosubstituted DNA