文章基本信息

标题：Studies on the Reverse Transcriptase of RNA Tumor Viruses. Structural Relatedness of Two Subunits of Avian RNA Tumor Viruses
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作者：Wade Gibson ; Inder M. Verma
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：12
页码：4991-4994
DOI：10.1073/pnas.71.12.4991
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The structural relationship of the small ([α]) and large ({beta}) subunits of reverse transcriptase isolated from two avian RNA tumor viruses has been examined by tryptic peptide analysis. Comparison of the tryptic hydrolysates of the isolated subunits by two-dimensional separation on thin-layer cellulose plates indicates that (i) the [α] subunit of reverse transcriptase of avian myeloblastosis virus is structurally related to the {beta} subunit; (ii) the [α] and {beta} subunits of the enzyme of Rous sarcoma virus also appear to be related; and (iii) there appears to be an extensive amino-acid sequence homology between reverse transcriptases of avian myeloblastosis virus and Rous sarcoma virus. Evidence is also presented that both [α] and {beta} subunits can be identified in purified avian myeloblastosis virions.
关键词：polyacrylamide gel electrophoresis ; in vitro radiolabeling with ¹²⁵I ; two-dimensional peptide analysis