文章基本信息

标题：The Regulation of Protein Synthesis in Mammalian Cells VI. Soluble and Polyribosome Associated Components Controlling In Vitro Polypeptide Initiation in HeLa Cells
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作者：Elliott S. Goldstein ; Marsha E. Reichman ; Sheldon Penman 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：12
页码：4752-4756
DOI：10.1073/pnas.71.12.4752
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The in vitro initiation of polypeptides on endogenous polyribosomes has been studied in extracts from HeLa cells. Regulation of the rate of initiation of polypeptides can be examined. In these experiments an assay using [35S]fMet-tRNAfMet has been developed, and the system further characterized. The system has been separated into a fraction containing polyribosomes with subunits and a fraction containing soluble components. The regulation of initiation has at least two distinct components. There is one factor in the soluble fraction which develops a stimulated response after protein synthesis has been inhibited in intact cells. This stimulation does not require new RNA synthesis during the period of cell "stress." A second component is associated with ribosomes. This factor is necessary for the initiation of polypeptides on endogenous polyribosomes. It disappears gradually when cells are exposed to actinomycin. The disappearance is first manifested by an inability of polyribosomes to respond to stimulated supernatants. This unstable component, which decays in the presence of actinomycin, has no apparent counterpart in systems that measure initiation on exogenous mRNA.
关键词：endogenous activity ; actinomycin inhibition ; in vivo regulation ; polyribosome initiation inhibition ; soluble initiation stimulation