文章基本信息

标题：Localization of Escherichia coli Ribosomal Proteins S4 and S14 by Electron Microscopy of Antibody-Labeled Subunits
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作者：James A. Lake ; Maryanne Pendergast ; Lawrence Kahan 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：12
页码：4688-4692
DOI：10.1073/pnas.71.12.4688
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Binding sites for antibodies specific for proteins S4 and S14 of the small subunit of E. coli ribosomes have been mapped on the surface of the subunit by electron microscopy. Antibody binding to reconstituted subunits was shown to depend specifically on the presence of E. coli S4 and S14. Anti-S14 IgG was found to bind to a limited region of the ribosome surface. In contrast anti-S4 IgG was found to bind to three separated regions of the ribosome surface, suggesting S4 has an elongated conformation in situ.
关键词：Bacillus stearothermophilus ; IgG antibody ; ribosome topography ; reconstitution