文章基本信息

标题：Differentiation of Lens-Like Structures from Newt Iris Epithelial Cells In Vitro
本地全文：下载
作者：Goro Eguchi ; Shin-Ichi Abe ; Kenji Watanabe 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：12
页码：5052-5056
DOI：10.1073/pnas.71.12.5052
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Dissociated cells of pigmented iris epithelium from adult newts grew intensively in monolayer cultures after a lag of two to three weeks. During the lag period, depigmentation occurred in many cells. When cultures became confluent five to six weeks after seeding, many tiny lens-like structures (30-70 per plate) differentiated from dense foci of amelanotic epithelial cells. These lens-like structures appeared in all cultures originated from cells of ventral as well as dorsal iris. The identification of these structures as lens was established by both immunological and ultrastructural techniques.
关键词：cytodifferentiation ; determination ; lens regeneration ; eye