文章基本信息

标题：A New Relaxed Mutant of Escherichia coli with an Altered 50S Ribosomal Subunit
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作者：J. D. Friesen ; N. P. Fiil ; J. M. Parker 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：9
页码：3465-3469
DOI：10.1073/pnas.71.9.3465
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A new relaxed mutant called rel C has been isolated from a rel A+/rel A+ partial diploid strain. The rel C mutant is unable to synthesize ppGpp or pppGpp in vivo in response to amino acid starvation or in vitro, but can synthesize these nucleotides in a shift-down. Rel C maps near rif. Studies in vitro demonstrate the lesion to be probably in one of the 50S ribosomal proteins that can be removed by 1.0 M LiCl.
关键词：RNA regulation ; guanosine tetraphosphate ; in vitro synthesis of ppGpp and pppGpp ; genetic mapping