文章基本信息

标题：Morphology of Nucleolar Cistrons in a Plant Cell, Acetabularia mediterranea
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作者：Michael F. Trendelenburg ; Herbert Spring ; Ulrich Scheer 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：9
页码：3626-3630
DOI：10.1073/pnas.71.9.3626
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The structural organization of transcriptionally active DNA that contains cistrons for precursor molecules of ribosomal RNA is described in positively stained spread preparations from nuclei and nucleoli isolated from the green alga, Acetabularia mediterranea Lmx. These nuclei contain large aggregates of nucleolar subunits in which fibril-covered regions, the putative active cistrons for precursors of ribosomal RNA, alternate with fibril-free intercepts, the "spacers." The length distribution of the different intercepts of this DNA is given, and the pattern is compared with those shown in animal cell systems. The data are discussed in relation to problems of transcription and of amplification of ribosomal RNA genes.
关键词：ribosomal RNA genes ; electron microscopy ; spread preparations