文章基本信息

标题：Quantitative Estimation of Agrobacterium tumefaciens DNA in Crown Gall Tumor Cells
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作者：Karl A. Drlica ; C. I. Kado
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：9
页码：3677-3681
DOI：10.1073/pnas.71.9.3677
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Several reports suggest that Agrobacterium tumefaciens nucleic acids can induce transformation of the cells of susceptible host plants and that bacteria-free tissue cultures of transformed cells contain A. tumefaciens DNA, RNA, antigens, or bacteriophages. We assayed Vinca rosea tumor DNA for base sequence homologies with A. tumefaciens DNA by DNA{middle dot}DNA solution enrichment and DNA{middle dot}DNA filter saturation hybridization techniques. No homologies were found by either method. The filter saturation hybridization technique included model filters containing known percentages of bacterial DNA mixed with V. rosea leaf DNA. Using this sensitive technique, we found that no more than 0.02% of the crown gall tumor genome could be homologous to A. tumefaciens DNA. This upper estimate of homology corresponds to 0.2 bacterial genome equivalent per diploid tumor cell.
关键词：DNA·DNA hybridization ; Vinca rosea ; callus culture