文章基本信息

标题：Prostaglandin Synthetase System—Resolution into Oxygenase and Isomerase Components
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作者：Tsumoru Miyamoto ; Shozo Yamamoto ; Osamu Hayaishi 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：9
页码：3645-3648
DOI：10.1073/pnas.71.9.3645
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The microsomal fraction of bovine vesicular gland catalyzed the conversion of [1-14C]8,11,14-eicosatrienoic acid to prostaglandin E1 in the presence of tryptophan, hemoglobin, and glutathione. The prostaglandin synthetase system was solubilized by treatment of the microsomal fraction with Tween 20 in the presence of ethylene glycol. DEAE-cellulose column chromatography separated the enzyme into two fractions (Fractions I and II), both of which were required for prostaglandin E1 synthesis. When Fraction I alone was incubated with 8,11,14-eicosatrienoic acid, an unstable compound accumulated. This compound was converted to prostaglandin E1 by the addition of Fraction II. On the basis of its RF values on thin-layer chromatography, its reduction to prostaglandin F1[α] with stannous chloride, and the decomposition to prostaglandins F1[α], E1, and D1 at room temperature, the unstable intermediate was tentatively identified to be the 9,11-endoperoxide derivative, referred to previously as prostaglandin R1 (Nugteren et al. (1973) Biochim. Biophys. Acta 326, 448-461) or prostaglandin H1 (Hamberg et al. (1974) Proc. Nat. Acad. Sci. USA 71, 345-349). Of the three cofactors mentioned above, Fraction I required both tryptophan and hemoglobin, while only glutathione was necessary for Fraction II. Anti-inflammatory agents such as indomethacin and aspirin were inhibitory to Fraction I.
关键词：unsaturated fatty acid ; endoperoxide ; glutathione ; hemoglobin ; indomethacin