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  • 标题:Direction of Transcription of the Regulatory Gene araC in Escherichia coli B/r
  • 本地全文:下载
  • 作者:Gary Wilcox ; James Boulter ; Nancy Lee
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1974
  • 卷号:71
  • 期号:9
  • 页码:3635-3639
  • DOI:10.1073/pnas.71.9.3635
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:The protein product of the regulatory gene araC can be synthesized in a cell-free, protein-synthesizing system programmed with a {lambda}paraC+B DNA template. Hybrid, renatured phage DNA molecules prepared with DNA from phages {lambda}paraC+B and {lambda}paraC3B (araC3 is a nonsense mutation) were used to program the cell-free synthesis of the araC protein. The findings observed lead to the conclusion that the codogenic strand of the araC gene is on the light strand of the phage DNA. The araB gene is on the heavy strand, as determined by DNA{middle dot}RNA hybridization. Thus, with regard to the standard E. coli map, araC is transcribed in a clockwise direction, whereas transcription of the araBAD operon has a counterclockwise orientation. The technique described should allow one to determine the direction of transcription of any gene that can be incorporated into the genome of a specialized transducing phage.
  • 关键词:protein synthesis in vitro ; clockwise transcription ; L-arabinose ; positive control
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