文章基本信息

标题：Nucleotide Sequence Analysis of RNA Synthesized from Rabbit Globin Complementary DNA
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作者：Raymond Poon ; Gary V. Paddock ; Howard Heindell 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：9
页码：3502-3506
DOI：10.1073/pnas.71.9.3502
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Rabbit globin complementary DNA made with RNA-dependent DNA polymerase (reverse transcriptase) was used as template for in vitro synthesis of 32P-labeled RNA. The sequences of the nucleotides in most of the fragments resulting from combined ribonuclease T1 and alkaline phosphatase digestion have been determined. Several fragments were long enough to fit uniquely with the [α] or {beta} globin amino-acid sequences. These data demonstrate that the cDNA was copied from globin mRNA and contained no detectable contaminants.
关键词：reverse transcriptase ; RNA polymerase ; globin mRNA