文章基本信息

标题：Use of Eukaryotic Native Small Ribosomal Subunits for the Translation of Globin Messenger RNA
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作者：Christoph Freienstein ; Günter Blobel
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：9
页码：3435-3439
DOI：10.1073/pnas.71.9.3435
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A highly active in vitro system for the translation of globin mRNA, resulting in more than 10 rounds of translation, is described. The reconstituted system consists of native small ribosomal subunits of rabbit reticulocytes (as a source of initiation factors as well as small ribosomal subunits), large subunits derived from rat liver polysomes by the puromycin-KCl procedure, and a pH 5 fraction obtained from a Krebs ascites cell high speed supernatant. In this system no differences were found between globin messenger ribonucleoprotein and globin mRNA.
关键词：rabbit reticulocytes ; rat liver polysomes ; Krebs ascites pH 5 supernatant