文章基本信息

标题：The 7S RNA Common to Oncornaviruses and Normal Cells is Associated with Polyribosomes
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作者：T. A. Walker ; N. R. Pace ; R. L. Erikson 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：9
页码：3390-3394
DOI：10.1073/pnas.71.9.3390
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The 7S RNA species first demonstrated in avian and murine oncornaviruses and later in normal, uninfected cells is found associated in part with cellular polyribosomes. A molar ratio of 7S RNA to 5S ribosomal RNA of 0.05 indicates that there is approximately one mole of 7S RNA per mole of messenger RNA. Dissociation of polyribosomes with dimethylsulfoxide results in a marked decrease in the sedimentation rate of the 7S RNA. The dimethylsulfoxide-induced dissociation of polyribosomes and the concomitant movement of the 7S RNA from the polyribosome region into lighter regions of a sucrose gradient are both inhibited by cycloheximide, indicating that the 7S RNA is indeed associated with polyribosomes and not with a ribonucleoprotein particle sedimenting with polyribosomes.
关键词：L-cells ; RNA fingerprinting ; gel electrophoresis ; dimethylsulfoxide