文章基本信息

标题：Replication of the Escherichia coli Chromosome with a Soluble Enzyme System
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作者：Thomas Kornberg ; Arthur Lockwood ; A. Worcel 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：8
页码：3189-3193
DOI：10.1073/pnas.71.8.3189
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Semi-conservative DNA synthesis is observed when the isolated, folded E. coli chromosome is supplemented with a DNA-free, soluble enzyme fraction, the four deoxynucleoside 5'-triphosphates, ATP, and Mg++. The DNA synthesized in vitro remains associated with the folded chromosome during sedimentation through neutral sucrose, but is released as small DNA fragments in alkali. Sealing of these replicative intermediates to the chromosome requires the presence of both E. coli DNA polymerase I (EC 2.7.7.7 ) and DNA ligase (EC 6.5.1.2 ).
关键词：spermidine ; folded DNA ; DNA polymerase I ; DNA ligase