文章基本信息

标题：Selective Toxicity of Diphtheria Toxin for Malignant Cells
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作者：Barbara H. Iglewski ; Marvin B. Rittenberg
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：7
页码：2707-2710
DOI：10.1073/pnas.71.7.2707
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Purified diphtheria toxin is shown to inhibit protein synthesis in Ehrlich-Lettre ascites carcinoma cells in vitro. Protein synthesis in Ehrlich-Lettre cells is at least 10,000 times more sensitive to toxin than protein synthesis in normal mouse spleen or thymus cells. This sensitivity correlates with the observation that Ehrlich-Lettre tumors regress in mice injected with diphtheria toxin but not diphtheria toxoid. Using the criterion of inhibition of protein synthesis in vitro, we show that other mouse malignancies (lymphoma and myeloma) are also more sensitive to diphtheria toxin than normal spleen or thymus. Metastatic human breast carcinoma cells from two individuals, cells from two melanoma nodules removed at different times from a third patient, and cells from melanoma nodules from three additional individuals are shown to be more sensitive to diphtheria toxin than some normal human cells. The toxin sensitivity of protein synthesis in some of the malignant cells tested was so much greater than that of normal cells, that we have proposed that diphtheria toxin should be studied further since it might prove a useful anti-cancer agent in patients whose tumors are first shown to be highly sensitive to toxin in vitro.
关键词：cancer ; protein synthesis