文章基本信息

标题：The Binding Characteristics and Number of β-Adrenergic Receptors on the Turkey Erythrocyte
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作者：Alexander Levitzki ; Daphne Atlas ; Michael L. Steer 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：7
页码：2773-2776
DOI：10.1073/pnas.71.7.2773
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Turkey erythrocyte ghosts (empty membranes) possess a class of receptors that can bind both L-[3H]isoproterenol and DL-[3H]propranolol. The binding of [3H]isoproterenol to these receptors occurs with a dissociation constant of 0.15 {micro}M and can be fully inhibited by 1 {micro}M propranolol. The binding of [3H]propranolol occurs with a dissociation constant of 2.5 nM and can be fully inhibited by 0.2 mM DL-isoproterenol. Ligand binding is sensitive to sonication, boiling, and 8 M urea. The cells possess 500 to 1000 {beta}-adrenergic receptors per cell. Binding of propranolol to the {beta}-receptor was found to be stereospecific for the L stereoisomer. If one assumed a 1:1 relationship between {beta}-adrenergic receptors and adenylate cyclase, the turnover number of this adenylate cyclase would be close to 100 min-1.
关键词：β-receptors ; isoproterenol ; propranolol ; equilibrium dialysis ; ultracentrifugation