文章基本信息

标题：Release of Polarity in Escherichia coli by Gene N of Phage λ: Termination and Antitermination of Transcription
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作者：Sankar Adhya ; Max Gottesman ; Benoit De Crombrugghe 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：6
页码：2534-2538
DOI：10.1073/pnas.71.6.2534
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The induction of {lambda} prophage provokes the constitutive expression of the adjacent gal operon in E. coli. This "escape synthesis" can result from transcription that initiates at a phage promoter and extends into the gal operon. The effect requires the product of the {lambda} gene N. N-mediated transcription not only fails to terminate at the prophage-bacterial junction and at the ends of bacterial operons, but ignores termination signals caused by polar insertions or ochre mutations within gal. Suppression of polarity by N-function is a cis-effect; only transcription initiated at the phage promoter is influenced. We propose that the transcription complex is influenced by N-product to become termination-resistant at a site in the phage genome (juggernaut model). This site appears to be at or near the phage promoter.
关键词：escape synthesis ; juggernaut model ; gal operon