文章基本信息

标题：Identification of Discrete Polyadenylate-containing RNA Components Transcribed from HeLa Cell Mitochondrial DNA
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作者：Deanna Ojala ; Giuseppe Attardi
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：2
页码：563-567
DOI：10.1073/pnas.71.2.563
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Polyacrylamide gel electrophoresis and sedimentation analysis under denaturing conditions of poly(A)-containing RNA from the polysome region of the sedimentation pattern of a HeLa-cell mitochondrial lysate has revealed the occurrence of a discrete RNA component, which sediments in the native state with a sedimentation constant of about 7 S. From the sedimentation behavior under native and denaturing conditions and the poly(A) content, a molecular weight of about 9 x 104 has been estimated for this component. RNA{middle dot}DNA hybridization experiments have indicated that this component is coded for by the light strand of mitochondrial DNA. Evidence for the occurrence of a poly(A)-containing RNA component sedimenting at about 9 S and coded for by the heavy strand has also been obtained.
关键词：actinomycin D ; denaturation ; poly(dT)-cellulose ; polyacrylamide gel ; separated strands ; RNA·DNA hybridization