文章基本信息

标题：Asymmetry in the 50S Ribosomal Subunit of Escherichia coli
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作者：P. B. Moore ; D. M. Engelman ; B. P. Schoenborn 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：1
页码：172-176
DOI：10.1073/pnas.71.1.172
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A substantial separation of the centers of mass of the protein and RNA portions of the 50S ribosomal subunit has been achieved using neutron scattering. This separation reveals that the subunit has a protein-rich side, a finding which is inconsistent with many models proposed for the structure. By analyzing the variation of the radius of gyration of the subunit under conditions in which the contributions of the protein and RNA were separately enhanced by deuteration and comparing these radii to the undeuterated subunit radius, we have obtained the following values. The radius of gyration of the whole particle is 78.0 {+/-} 0.95 A, that of the RNA moiety is 72.5 {+/-} 1.5 A, and that of the protein is 73.4 {+/-} 2.0 A. These data give a separation of the centers of mass of the RNA and protein distributions of 57.7 {+/-} 10 A.