文章基本信息

标题：Involvement of Two Protein Factors and ATP in In Vitro DNA Synthesis Catalyzed by DNA Polymerase III of Escherichia coli
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作者：Jerard Hurwitz ; Sue Wickner
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：1
页码：6-10
DOI：10.1073/pnas.71.1.6
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The in vitro conversion of single-stranded DNA from bacteriophage fd to duplex structures depends on E. coli RNA polymerase, DNA polymerase III, riboand deoxyribonucleoside triphosphates, Mg+2, spermidine or DNA-unwinding protein of E. coli, and two additional protein factors, referred to here as Factors I and II. These two factors are also essential for dTMP incorporation catalyzed by DNA polymerase III and dependent on poly(dA){middle dot}oligo(dT) primer-template. In the latter reaction, there is an absolute dependency on ATP or dATP.
关键词：DNA replication ; fd DNA ; poly(dA)·oligo(dT)