文章基本信息

标题：Cytogenetic Engineering In Vivo: Restoration of Biologic Complement Activity to C5-Deficient Mice by Intravenous Inoculation of Hybrid Cells
本地全文：下载
作者：Nelson L. Levy ; Ralph Snyderman ; Roger L. Ladda 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：11
页码：3125-3129
DOI：10.1073/pnas.70.11.3125
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Splenic macrophages were identified as at least one source of C5 elaboration in normal mice. Hybrid cells were formed from splenic macrophages from C5-deficient mice and either kidney cells from mice with normal amounts of C5 or chicken erythrocytes. These hybrids elaborated C5 in vitro. C5-Deficient mice inoculated with these hybrid cells developed, in their serum, antigenically active mouse C5, as well as both hemolytic and biologic complement activity. These studies demonstrate the feasibility of genetic "repair" in mammals.
关键词：mouse splenic macrophages ; chicken erythrocytes ; mouse kidney cells ; genetic repair ; MuB1 protein