文章基本信息

标题：Relation of Detergent HLB Number to Solubilization and Stabilization of D-Alanine Carboxypeptidase from Bacillus subtilis Membranes
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作者：Jay N. Umbreit ; Jack L. Strominger
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：10
页码：2997-3001
DOI：10.1073/pnas.70.10.2997
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The ability of various nonionic detergents to solubilize D-alanine carboxypeptidase and other membrane-bound enzymes was correlated to a physical property of the detergent, the HLB number. Only a fairly narrow range of detergents were effective solubilizing agents. Purified carboxypeptidase required either a detergent or detergent plus a lipid fraction for stability. Only those detergents effective in solubilizing the enzyme were effective in stabilizing it.
关键词：hydrophobic lipophilic balance ; nonionic detergents ; succinate dehydrogenase ; phosphoacetylmuramyl-pentapeptide translocase