文章基本信息

标题：Enzyme Loading of Erythrocytes
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作者：Garret M. Ihler ; Robert H. Glew ; Frederick W. Schnure 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：9
页码：2663-2666
DOI：10.1073/pnas.70.9.2663
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We demonstrated that {beta}-glucosidase and {beta}-galactosidase can be trapped inside erythrocytes by rapid hemolysis of the cell in the presence of these enzymes. Enzyme enters only during hemolysis, and optimum uptake occurs within 60 sec. There is no loss in cell number after hemolysis-induced enzyme uptake, and the ghosts have only a slightly increased mean cell volume. Smaller proteins enter more readily than larger proteins, although enzymes with a molecular weight of at least 180,000 can be readily entrapped by erythrocytes. This finding may provide a useful approach to the problem of enzyme replacement in certain diseases, including Gaucher's disease.
关键词：sphingolipidoses ; Gaucher's disease ; β-glucocerebroside ; β-glucocerebrosidase ; spherocytes