文章基本信息

标题：Transformation of Neuraminidase-Treated Lymphocytes by Soybean Agglutinin
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作者：Abraham Novogrodsky ; Ephraim Katchalski
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：9
页码：2515-2518
DOI：10.1073/pnas.70.9.2515
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Transformation of mouse-spleen lymphocytes in the presence of soybean agglutinin is markedly enhanced after their treatment with neuraminidase (EC 3.2.1.18 ). Incubation of the cells with the enzyme also facilitates their agglutination by the lectin. The soybean agglutinin-induced agglutination and transformation is inhibited specifically by N-acetyl-D-galactosamine. New specific binding sites for soybean agglutinin were shown to appear after neuraminidase treatment. It is postulated that the transformation of neuraminidase-treated mouse-spleen lymphocytes induced by soybean agglutinin is caused by the binding of the lectin to galactosyl residues exposed by the action of neuraminidase on the cell membrane.
关键词：mouse spleen ; agglutination ; membrane receptors ; cell lines