文章基本信息

标题：Molecular Structures of Acetylcholinesterase from Electric Organ Tissue of the Electric Eel
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作者：Yadin Dudai ; Max Herzberg ; Israel Silman 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：9
页码：2473-2476
DOI：10.1073/pnas.70.9.2473
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Three purified molecular forms of acetylcholinesterase (EC 3.1.1.7 ) with sedimentation coefficients of 18 S, 14 S, and 11 S were studied by analytical ultracentrifugation and electron microscopy. The three species have molecular weights of (1.1 {+/-} 0.1) x 106, (7.5 {+/-} 1.5) x 105, and (3.35 {+/-} 0.25) x 105, respectively. Electron micrographs reveal that the 18S and 14S forms are asymmetric, composed of a head, containing a large number of subunits, and an elongated tail. The 11S form of acetylcholinesterase is apparently a tetrameric structure devoid of the tail. Maleylation of 18S and 14S acetylcholinesterases abolishes their tendency to aggregate at low ionic strength.
关键词：electron microscopy ; analytical ultracentrifugation ; maleylation ; protein subunits ; membrane enzymes