文章基本信息

标题：A DNA-Binding Protein Induced by Bacteriophage T7
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作者：Roberta C. Reuben ; Malcolm L. Gefter
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：6
页码：1846-1850
DOI：10.1073/pnas.70.6.1846
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A DNA-binding protein has been purified from Escherichia coli infected with bacteriophage T7 by DNA-cellulose chromatography. The protein is absent in uninfected cells. The purified protein has a molecular weight of 31,000 and binds strongly and preferentially to single-stranded DNA. In vitro studies show that this protein can stimulate the rate of polymerization catalyzed by the T7-induced DNA polymerase 10-15 times under conditions where the polymerase is unable to effectively use a single-stranded template. The degree of stimulation is dependent upon the ratio of binding protein to DNA template and is independent of polymerase concentration. The observed stimulation is specific for the T7 DNA polymerase in that addition of the protein to reactions catalyzed by E. coli DNA polymerases I, II, or III or T4 DNA polymerase is without effect.
关键词：DNA cellulose ; T7 DNA polymerase ; stimulation of synthesis