文章基本信息

标题：A Temperature-Sensitive Change in the Energy of Activation of Hormone-Stimulated Hepatic Adenylyl Cyclase
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作者：Peter W. Kreiner ; James J. Keirns ; Mark W. Bitensky 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：6
页码：1785-1789
DOI：10.1073/pnas.70.6.1785
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We describe an abrupt increase (at 32{degrees}) in the energy of activation for the reaction of hepatic adenylyl cyclase in the presence of glucagon or epinephrine. This increase is not seen in the presence of fluoride, prostaglandin E1, or 1-propanol, or in the absence of cyclase stimulators. The change in energy of activation found with hormones is abolished by 1-propanol. This change does not represent differences in hormone or substrate binding at different temperatures, but seems to reflect interactions among elements of the cyclase stimulation sequence. Similar changes in energy of activation were not observed for alkaline phosphatase, cyclic AMP-phosphodiesterase, 5'-nucleotidase, or ouabain-sensitive ATPase. Since the mole fraction of cholesterol in liver membranes is sufficiently high to preclude a phase change in bulk membrane lipids, our observation suggests either that cyclase is restricted to cholesterol-poor membrane regions or that the change in its energy of activation is largely restricted to protein components of the cyclase apparatus. The data are compatible with fundamental differences in the stimulation process(es) for the hormones (glucagon and epinephrine) as compared with those for fluoride and prostaglandin E1.
关键词：epinephrine ; glucagon ; prostaglandin E₁ ; fluoride