文章基本信息

标题：A New Form of DNA Polymerase III and a Copolymerase Replicate a Long, Single-Stranded Primer-Template
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作者：William Wickner ; Randy Schekman ; Klaus Geider 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：6
页码：1764-1767
DOI：10.1073/pnas.70.6.1764
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A new form of DNA polymerase III, termed Pol III star (Pol III*), has been purified to homogeneity from Escherichia coli. Pol III* is temperature sensitive when isolated from a thermo-sensitive dnaE mutant, as had been described for Pol III. Pol III* and Pol III are separable by gel filtration. Pol III* utilizes a duplex template containing short gaps with the same catalytic properties as Pol III. However, Pol III* is able to replicate long, singlestranded templates such as homopolymer chains and viral circles of M13 and {phi}X174 if provided with the following: spermidine, a primer fragment, and a new protein, termed copolymerase III* (Copol III*). The latter, purified to homogeneity, has no known independent enzymatic activity and supports synthesis by Pol III* but not by Pol I, Pol II, or Pol III.
关键词：DNA replication ; M13 ; ϕX174 ; spermidine ; dna E mutant