文章基本信息

标题：Studies on In Vitro DNA Synthesis Purification of the dna G Gene Product from Escherichia coli
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作者：Sue Wickner ; Michel Wright ; Jerard Hurwitz 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：5
页码：1613-1618
DOI：10.1073/pnas.70.5.1613
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：{phi}X174 DNA-dependent dNMP incorporation is temperature-sensitive (ts) in extracts of uninfected E. coli dna A, B, C, D, E, and G ts strains. DNA synthesis can be restored in heat-inactivated extracts of various dna ts mutants by addition of extracts of wild-type or other dna ts mutants. A protein that restores activity to heat-inactivated extracts of dna G ts cells has been extensively purified. This protein has also been purified from dna G ts cells and is thermolabile when compared to the wild-type protein. The purified dna G protein has a molecular weight of about 60,000, is insensitive to N-ethylmaleimide, and binds poorly to DNA. It does not stimulate heat-inactivated crude extracts of dna B, C, D, or E ts cells and lacks detectable RNA and DNA polymerase activities.
关键词：dna A, dna B, dna C, dna D, and dna E gene products ; ϕX174 ; DNA replication ; DNA polymerase III