文章基本信息

标题：Conformations of Diphosphopyridine Coenzymes upon Binding to Dehydrogenases
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作者：Chi-Yu Lee ; Ronald D. Eichner ; Nathan O. Kaplan 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：5
页码：1593-1597
DOI：10.1073/pnas.70.5.1593
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The binding of oxidized as well as reduced coenzyme to some dehydrogenases has been studied under different concentration ratios and temperatures by nuclear magnetic resonance spectroscopy. A significant difference in the spectral behavior between DPN+ and DPNH upon binding is interpreted in terms of fast and slow on-off rates relative to the nuclear magnetic resonance time scale in the binding of these two coenzymes. Significant downfield shifts of DPN+ were observed upon binding, comparable in magnitude to those expected upon opening (destacking) of the coenzymes in the case of chicken-muscle and lobster-tail lactate dehydrogenase (EC 1.1.1.27 ) and yeast alchol dehydrogenase (EC 1.1.1.1 .). A preliminary survey of several other dehydrogenases is consistent with these findings. In the case of 3-phosphoglyceraldehyde dehydrogenase, there is a possibility that the coenzyme exists in the folded form.
关键词：cofactor-enzyme interaction ; NMR spectroscopy ; kinetics of binding