文章基本信息

标题：Papain-Solubilized HL-A Antigens from Cultured Human Lymphocytes Contain Two Peptide Fragments
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作者：Peter Cresswell ; Mervyn J. Turner ; Jack L. Strominger 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：5
页码：1603-1607
DOI：10.1073/pnas.70.5.1603
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：HL-A 2 and HL-A 7 antigens have been solubilized from cultured human lymphocytes by papain and purified on a small radioactive scale after formation of immune complexes, or on a large scale by column chromatographic methods. In both cases, it has been shown that the materials obtained consist of two noncovalently bound fragments. One fragment is a glycopeptide of molecular weight 30,000-31,000, the other is a peptide of molecular weight 11,000-12,000. In polyacrylamide gels containing 8 M urea, the large fragments of HL-A 2 and HL-A 7 exhibited different electrophoretic mobilities, while the small fragments were the same. The relationship of these materials to the native HL-A molecule is discussed.
关键词：histocompatibility ; glycoproteins ; membrane ; molecular weight