文章基本信息

标题：Topographical Location of H—Y Antigen on Mouse Spermatozoa by Immunoelectronmicroscopy
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作者：Gloria C. Koo ; Christopher W. Stackpole ; Edward A. Boyse 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：5
页码：1502-1505
DOI：10.1073/pnas.70.5.1502
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：H-Y (male) antigen was visually located on mouse sperm by electron microscopy, by use of the indirect hybrid antibody method and tobacco mosaic virus as the visual marker. Labeling was achieved by centrifugation of the sperm through a discontinuous gradient consisting of alternating layers of immune reagents and wash solutions. Treated sperm were examined topographically by preparation of platinum-carbon replicas. Antigen was located mainly on the acrosomal cap of the sperm head.
关键词：hybrid antibodies ; tobacco mosaic virus ; gradient centrifugation ; replicas