文章基本信息

标题：Effect of Hemin on the Synthesis of Hemoglobin and Other Proteins in Mammalian Cells
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作者：Yves Beuzard ; Robert Rodvien ; Irving M. London 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：4
页码：1022-1026
DOI：10.1073/pnas.70.4.1022
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The initiation of globin synthesis in intact reticulocytes and in reticulocyte lysates is maintained by the addition of hemin. The specificity of this effect has been studied to determine whether it is restricted to hemoglobin and erythroid cells. In intact reticulocytes, hemin (500 {micro}M) enhances the synthesis of carbonic anhydrase as well as of hemoglobin. Similar enhancement of protein synthesis is observed on addition of hemin (500 {micro}M) to intact Krebs II ascites tumor cells, in cell-free extracts of these cells, added hemin (50 {micro}M) increases endogenous protein synthesis and the translation of exogenous rabbit globin messenger RNA. These results provide evidence that the effect of hemin is not restricted to globin, and they suggest that hemin may enhance protein synthesis in at least some nonerythroid cells.
关键词：carbonic anhydrase ; Krebs II ascites tumor cells ; reticulocytes