文章基本信息

标题：Chromosome Structure as Revealed by a Combined Chemical and Immunochemical Procedure
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作者：R. R. Schreck ; D. Warburton ; O. J. Miller 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：3
页码：804-807
DOI：10.1073/pnas.70.3.804
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Human metaphase chromosomes were photooxidized in the presence of methylene blue, a process that destroys guanine residues in DNA. Indirect immunofluorescence showed that such chromosomes reacted with a cytosine-specific antibody revealing a consistent fluorescent banding pattern by which each chromosome could be identified. The observed fluorescent patterns were the reverse of those produced in formamide-denatured chromosomes treated with an antibody specific for adenine and of the patterns obtained with quinacrine and with Giemsa staining by the G-banding techniques. The patterns were identical to Giemsa R-banding patterns. The chromosome banding patterns, therefore, appeared to reflect DNA base composition, indicating the feasibility of a combined chemical-immunochemical investigation of the chemical organization of chromosomes.
关键词：photooxidation ; cytosine-specific antibodies ; chromosome banding ; immunofluorescence