文章基本信息

标题：Isolation of the Self-Regulated Repressor Protein of the Hut Operons of Salmonella typhimurium
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作者：David C. Hagen ; Boris Magasanik
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：3
页码：808-812
DOI：10.1073/pnas.70.3.808
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：In Salmonella typhimurium the structural genes of the enzymes responsible for histidine utilization (hut) are clustered in two adjacent operons. A single repressor regulates both operons. The repressor itself is a member of one of the hut operons and, thus, regulates its own synthesis. We have assayed the hut repressor by its ability to bind radioactive DNA to nitrocellulose filters. The binding is specific for DNA bearing the hut operons, and the binding is abolished by the inducer, urocanate. As a member of one of the hut operons, the repressor is inducible, subject to catabolite repression, and affected by a promoter mutation.
关键词：histidine utilization ; DNA-binding ; urocanate ; λp hut