文章基本信息

标题：Microtubule Assembly in the Absence of Added Nucleotides
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作者：Michael L. Shelanski ; Felicia Gaskin ; Charles R. Cantor 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：3
页码：765-768
DOI：10.1073/pnas.70.3.765
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Microtubule assembly is enhanced by the addition of 1 M sucrose or 4 M glycerol to the reassembly mixture. Tubulin can be purified from guinea pig brain readily and in good yield by two cycles of assembly in glycerol-containing solutions. The tubules assembled in glycerol and sucrose are more stable than tubules formed in the absence of these compounds. Assembly occurs in glycerol or sucrose in the absence of ATP or GTP, but is greatly accelarated by their presence.
关键词：sucrose ; glycerol ; tubulin ; guinea pig brain ; GTP