文章基本信息

标题：Inhibition of 5-Oxoprolinase by 2-Imidazolidone-4-Carboxylic Acid
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作者：Paul Van Der Werf ; Ralph A. Stephani ; Marian Orlowski 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：3
页码：759-761
DOI：10.1073/pnas.70.3.759
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：L-2-Imidazolidone-4-carboxylic acid is an effective competitive inhibitor of the reaction catalyzed by 5-oxoprolinase, in which 5-oxo-L-proline (L-pyroglutamic acid, L-2-pyrrolidone-5-carboxylic acid, L-5-oxopyrrolidine-2-carboxylic acid) is converted to L-glutamate, with concomitant cleavage of ATP to ADP and orthophosphate. L-2-Imidazolidone-4-carboxylate decreased the rate of metabolism of 5-oxo-L-[14C]proline to 14CO2 by rat-kidney slices but had no effect on the metabolism of [14C]glutamate. Mice injected with L-2-imidazolidone-4-carboxylate exhibited greatly reduced ability to metabolize 5-oxo-L-proline, but metabolized glutamate at an essentially normal rate. The findings provide an approach to an animal model for the human condition 5-oxoprolinuria, in which there is apparently a deficiency of renal 5-oxoprolinase activity. The evidence indicates that 5-oxoproline is a normal metabolite.
关键词：pyroglutamate ; pyrrolidone carboxylate ; glutathione ; γ-glutamyl cycle ; kidney ; aminoacid transport