文章基本信息

标题：Microinjection of Rabbit Hemoglobin Messenger RNA into Amphibian Oocytes and Embryos
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作者：J. Brachet ; G. Huez ; E. Hubert 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：2
页码：543-547
DOI：10.1073/pnas.70.2.543
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：After microinjection of hemoglobin mRNA from rabbit reticulocytes (9S mRNA) into Xenopus laevis oocytes, the cytological structure remains normal. The peroxidase reaction does not show an accumulation of synthesized hemoglobin in the germinal vesicle. Fertilized Pleurodeles Waltlii eggs synthesize rabbit hemoglobin after injection of 9S mRNA; hemoglobin synthesis decreases as development progresses. Micromeres and macromeres synthesize equal amounts of hemoglobin, despite the fact that the micromeres contain many more ribosomes. Descendants of injected blastomeres divide more slowly and do not differentiate; they ultimately undergo cytolysis.
关键词：9S RNA ; globin mRNA ; Xenopus oocytes ; Pleurodeles embryos